Jeg3 hla-g
Web1 apr 2024 · Furthermore, CRISPR/Cas9-mediated deletion of Enhancer L in the trophoblast-like HLA-G + JEG3 cell line and in primary EVT revealed that it is absolutely required for HLA-G expression. Interestingly, CTCF binding sites immediately upstream of Enhancer L and downstream of the HLA-G coding sequence likely establish an insulated … WebFig. 5 HLA-G shedding in JEG3 cell lines. Evaluation of JEG3 during a time point experiment (2, 6, 24 h) without or with MMP-2 or ... HLA-G has a concentration-dependent effect on the generation of an allo-CTL response. …
Jeg3 hla-g
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WebWe designed oligonucleotides to interfere exclusively with HLA-G mRNA, which were applied at different concentrations to 50% confluent Jeg-3 cells. After 36 hr, the HLA-G … Web15 mar 2024 · JEG-3 (HTB-36™, American Type Culture Collection) is an epithelial-like choriocarcinoma cell line with an EVT-like phenotype (KRT7+GATA3+TFAP2C+HLAG+) (19). It was cultured in DMEM/F12 supplemented with 10% fetal bovine serum (FBS). 2.2 Establishment of the JEG-3 organoids and generation of EVT cells
Web15 gen 2024 · 1. Introduction. In the early seventies the clonal cell line JEG-3 was established from a patient-derived choriocarcinoma after long-term passaging in the … Web2 giu 2024 · In our DAUI example, no HLA-G transcripts can be detected (Table 6a) thereby preventing HLA-G typing (Table 5a). Similarly, in our JEG-3 example, HLA-DRB1 is the only locus for which seq2HLA was able to determine the HLA ... DAUDI_SRR387401-ClassII.expression and b) JEG-3(SRR3317028) stored in JEG3_SRR3317028-ClassI …
Web13 apr 2024 · The proliferation ability, migration, and invasion ability were detected by CCK8 assay (Fig. 3C), Edu staining (Figs. 3D, and E), and wound healing (Figs. 3F, and G). Given the large cell size of JEG3, the cell chamber was difficult to penetrate. Thus, the invasion ability of JEG3 after miR-526b-5p knockdown was not detected. WebI ’uéä’BI ™C¦Jé!’BIX’R»$% Rd‘&P隤¢‰²P“+$Ê´VÀ¼ ëF´g¹–\í\,ôl ©jæi eU)ðº 8×Cq-Ä™X’BI ™,Üè»ñ•ÊH ¨6vj¾kã¤Ú‰%I&‡MO6 3a; VÕ ·A`¯Æ“i Uë˜ Ô㤑Ó%’HtÈI*vI JT’ J %*gtŠ# c4¡N¬f“‘RD]1'Ηsž¤ ÙM š Z&%Knø© fB$‘ u` «=¤ VC/O í&PXé,× \Ù÷ júÛy,¦½iyA2õ*¾q } ¼Ý[;cx ë}}y*Íõ¥äŽzÛù#G®? 5{ b^
WebHLA-G gene transcription was determined by real-time PCR while HLA-G translation was investigated by a specific enzyme-linked immunosorbent assay for HLA-G and western …
Web1 nov 2003 · Like IFN, the lymphokines IL-10 and LIF are also reported to enhance HLA-G surface expression on JEG3 or trophoblast cells 33, 34. We did not obtain a substantial increase of HLA-G5 specific spots in response to these agents. marianne chapman obituaryWebInvitrogen Anti-HLA-G Monoclonal (87G), Catalog # MA1-10356. Tested in Immunohistochemistry (Frozen) (IHC (F)), Flow Cytometry (Flow), ELISA ... Human Jeg3 cells positive for HLA-G1 were used as a positive control. Unstained cells in dark grey were compared to anti-HLA-G labeled cells (clone 87G) shown as black line. Gates were … custodiamo il turismo in pugliaWeb24 mar 2011 · HLA-G gene transcription was determined by standard and real-time RT-PCR analysis, and protein expression was evaluated by both flow cytometry and Western … custodiamo le imprese bandoWebMethods Using an MS2-tethering approach in combination with small RNA sequencing, a number of putative miRNAs binding to the CDS of human leukocyte antigen (HLA)-G were identified. These candidate miRNAs were extensively screened for their effects in the HLA-G-positive JEG3 cell line. custodiamo le imprese 2.0WebIntroduction. HLA-G is a non-classical class I HLA molecule with limited polymorphism in its coding region. Multiple isoforms of this unique gene are highly expressed throughout pregnancy in fetal placental cells that embed deeply into the maternal decidua, invading the spiral arteries and directly contacting maternal immune cells (reviewed in Le Bouteiller et … custodiamo le imprese 2022WebG@ Bð% Áÿ ÿ ü€ H FFmpeg Service01w ... marianne chardomeWeb7 mar 2024 · JEG3 were collected 48 h posttransfection by trypsin treatment, and GFP-expressing cells were sorted on a FACSAria III (BD). GFP+ JEG3 were plated on 10-cm culture dishes at 5000 cells/dish and allowed for recovery for 10 days. Single colonies were manually picked and dispersed and replated individually into 96-well plates. marianne chapman lmhc