Flpin cho
WebFlp-In™-CHO 细胞系. Flp-In™ 细胞系设计用于快速生成稳定细胞系以从 Flp-In™ 表达载体表达关注的蛋白。. 这些细胞在转录活性基因组基因座处包含一个稳定整合的 FRT 位点。. Flp-In™ 表达载体的靶向整合可确保您的目的基因高水平表达。. 有六种 Flp-In™ 细胞系可 ... WebThe Flp-In™-CHO Cell Line was created by transfecting CHO cells with pFRT/ lac Zeo2 and selecting for Zeocin™-resistant clones. The Flp-In™ T-REx™-293 Cell Line contains …
Flpin cho
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Webcompounds NECA and VCP746 (1) in A2AR-FlpIn-CHO cells; n=4. Figure S81. Subtype selectivity study, cAMP accumulation stimulated by the bitopic compounds 3, 6, 8, 10, 13, 16 in A2AR-FlpIn-CHO cells; n=4. NECA (blue) and VCP746 (red) shown as dotted curves. S45 Figure S82. Subtype selectivity study, cAMP accumulation stimulated by the reference WebOct 1, 2024 · FlpIn-CHO cells stably transfected with human c-myc-GLP-1R cells were seeded at a density of 30,000 cells/well into clear 96-well culture plates and incubated overnight for 30 min at 37 °C in 5% CO 2, and serum-starved in FBS-free DMEM for 6 h prior to being assayed.
WebThe Flp-In System streamlines the generation of stable mammalian expression cell lines by taking advantage of a Saccharomyces cerevisiae-derived DNA recombination system. … WebItem Flp-In-CHO Cell Line; Company Thermo Fisher Scientific; Catalog Number R75807; This product is no longer available on Biocompare. Biocompare is the leading resource …
WebCreating vocal chops in FL Studio: step-by-step. 1. Open up Slicex and drag and drop your vocal into the plug-in, or load your sample by hitting Ctrl+O and selecting it via the … WebMar 31, 2024 · FlpIn-CHO cells stably expressing hRXFP3-Rluc8 or hRXFP4-Rluc8 and either β-arrestin-1-Venus or β-arrestin-2-Venus were seeded into white plates at a density of 30,000 cells/well and incubated...
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WebNov 4, 2024 · The Flpin-CHO-K1 cells that are engineered to stably express human CB2 receptor (the Flpin-CHO-CB2 cells) were cultured in the growth medium (Ham’s F12K + 10%FBS + 1×Ps + 800 ug/mL HB) at 37 °C and 5% CO 2. The cells were then collected by centrifugation and resuspended in Hank’s balanced salt solution (HBSS), 0.1% bovine … green flag customer services emailflushed woerdenWeb一站式科研服务平台. 学术工具. 文档翻译; 收录引证; 论文查重; 文档转换 flushed woerden reviewsWebObjective To construct a double transfected Flp-In TM CHO cell line stably expressing both cytochrome P450 family 2 subfamily A member 13(CYP2A13) and multidrug resistance … flushed with nitrogenWebFlpIn CHO hM 1 cells were transfected with 2 μg of pcDNA3L-His-CAMYEL. 24 h post-transfection cells were plated in 96-well assay plates and grown overnight. The assay was started by adding 10 μl of the cell-permeant substrate specific for Renilla luciferase (Rluc), coelenterazine h, to the well to yield a final concentration of 5 μ m . flushed 翻译WebApr 25, 2014 · Vector for constitutive secretion of BG505 SOSIP.664 gp140 in a Flp-In™ based expression system, and stable cell line selection. (A) Design of the pAM/C construct for expressing BG505 SOSIP.664 gp140. The plasmid map shows the site of the env and furin gene insertions, the promoters and the Poly A sequences.(B) Intracellular Env … green flag customer service ukWebAll of the Flp-In™ cell lines (except Flp-In™-CHO™; see the following section) contain a single integrated FRT site and stably express the lacZ-Zeocin™ fusion gene from the … flushed with the wall