Dynamic phototuning of 3d hydrogel stiffness
WebSep 5, 2024 · MDA-MB-231 triple negative breast carcinoma cells cultured in a 3D alginate-based hydrogel system displayed a stiffness-dependent response to the chemotherapeutic doxorubicin. ... we utilized a platform capable of dynamic ECM stiffness modulation to allow for a change in matrix stiffness over time. ... Allen S, Suggs L. Dynamic … WebSep 5, 2024 · Hydrogel stiffness was determined by calculating Young's modulus from frequency sweep measurements obtained from a rheometer (B). Figure 2. MDA-MB-231 cells have a stiffness-dependent resistance to doxorubicin. ... Suggs L. Dynamic phototuning of 3D hydrogel stiffness. Proc Natl Acad Sci USA. (2015) 112:1953–8. …
Dynamic phototuning of 3d hydrogel stiffness
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WebNational Center for Biotechnology Information WebDynamic phototuning of 3D hydrogel stiffness [Applied Biological Sciences] Hydrogels are widely used as in vitro culture models to mimic 3D cellular microenvironments. The …
WebHydrogels are widely used as in vitro culture models to mimic 3D cellular microenvironments. The stiffness of the extracellular matrix is known to influence cell phenotype, inspiring work toward unraveling the role of stiffness on cell behavior using hydrogels. However, in many biological processes such as embryonic development, … WebMar 8, 2024 · This system was effective to regulate fibroblast morphology based on dynamic phototuning of stiffness and demonstrated to be …
WebFeb 2, 2015 · Here, we present a 3D hydrogel cell culture system in which the matrix stiffness can be tuned by light. Our approach offers both spatial and temporal control of … WebDec 1, 2024 · While numerous studies have investigated the cellular response to static material stiffness on 2D and in 3D matrices, there are only few reports on the effect of dynamic mechanical cues on encapsulated cells in biomaterials [33, 34].In these studies, however, the number of dynamic stiffness change cycles were limited and the range of …
WebOct 10, 2024 · The low stiffness of the hydrogels combined with the user-defined adaptability of soft DNA interactions can be particularly suited to address cellular response in 3D matrices, for example, using cell culture grown as spheroids. 16 The thermosensitive response of the PIC polymers 6 induces polymer bundling resulting in hydrogels with …
WebMay 30, 2024 · Here, we utilize a dynamic 3D hydrogel capable of dynamic stiffening to interrogate the role of matrix stiffening on the invasive transformation of mammary epithelial cells. ... Dynamic phototuning of 3D hydrogel stiffness. PNAS. 2015; 2: 201421897. Google Scholar. OpenURL Placeholder Text cityhill onlineWebHow- ever, no cell culture systems exist to appropriately model temporal changes in ECM stiffness to determine the biological relevance and mechanisms involved. Here, we … did beatrix potter ever marryWebFeb 2, 2015 · Here, we present a 3D hydrogel cell culture system in which the matrix stiffness can be tuned by light. Our approach offers both spatial and temporal control of … did beatrice arthur have childrenWebAug 15, 2024 · The hydrogel with dynamic increased stiffness was prepared with methacrylated gelatin (GelMA)/sodium alginate (SA) containing CaCO 3 and D-(+) … cityhill.orgWebJan 12, 2024 · High extracellular matrix stiffness is a prominent feature of malignant tumors associated with poor clinical prognosis. To elucidate mechanistic connections between … city hills builders corporationWebFeb 17, 2015 · Temporal modulation of hydrogel stiffness is a powerful tool that will enable investigation of the role that dynamic microenvironments play in biological processes … city hill online storeWebHere, we present a 3D hydrogel cell culture system in which the matrix stiffness can be tuned by light. Our approach offers both spatial and temporal control of stiffness, is … city hill middle school naugatuck ct