Biotin-coupled mirna capture
WebFeb 27, 2013 · Biotin-coupled miRNA capture Biotin-labelled miRNAs were transfected into HEK293 cells at a final concentration of 20 nM. Forty-eight hours after transfection, cells were collected and treated as ... WebJun 25, 2024 · Biotin-coupled miRNA capture. The biotin-coupled miRNA pull-down assay was performed using previously reported method 8. Briefly, the 3′-end biotinylated …
Biotin-coupled mirna capture
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WebMay 31, 2024 · Biotin-coupled miRNA capture The biotin-coupled miRNA pull-down assay was performed as described previously by Zheng and colleagues ( 16 ). Briefly, the 3′ end biotinylated miR-RNA mimic or control biotin-RNA (RiboBio) was transfected into SPC-A1 cells at a final concentration of 20 nmol/L for 1 day. WebNov 9, 2024 · On the contrary, some studies argued that biotin moiety when attached to the 3′ end of the miRNA impede its association with AGO2. 41 Hence, biotin-labelled miRNA was not co-IPed with AGO2. 28 The negative result could be due to low transfection efficiency or post-translational modification of AGO2 that can hamper miRNA-AGO2 …
WebJan 31, 2024 · The biotin-coupled RNA complex was pull-downed by incubating the cell lysates with streptavidin magnetic beads (Life Technologies, USA) for another 4 h. The … WebFeb 19, 2024 · Provided herein are methods of identifying the spatial location of a nucleic acid in a biological sample. In some embodiments, the methods employ a template switching oligonucleotide. In some embodiments, the methods extend the capture probe to create a complementary DNA (cDNA) molecule of a capture analyte and produce second strand …
WebJul 17, 2024 · Biotin-coupled miRNA capture. Because PTEN plays an important role in regulating cell proliferation and is one of the miR-181b-5p target genes, a pull-down assay was performed to investigate whether miR-181b-5p directly binds to PTEN. Briefly, HPDLSCs were transfected with biotinylated miRNA mimics or nonsense control (NC) … WebJul 13, 2024 · 2.13 Biotin-coupled miRNA capture. The biotin-coupled miRNA pull-down assay was carried out as previously described. 17, 18 Briefly, the 3′-end biotinylated miR-RNA mimic or control biotin-RNA (RiboBio) was transfected into SPCA1 cells at a final concentration of 20 nmol/L for 1 day. The biotin-coupled RNA complex was pulled …
WebMiR-4306 response elements were predicted by bioinformatics algorithm and identified using dual-luciferase reporter, RNA immunoprecipitation, and biotin-coupled miRNA capture assays. Results CircPDE4D was markedly downregulated in OA cartilages and interleukin (IL)-1β-stressed human normal chondrocytes (HNC).
WebFeb 10, 2024 · Biotin-coupled miRNA capture. The 3′ end biotinylated miR-578 or control mimic RNA (RiboBio) was transfected into RF/6A cells for 24 h at the concentration of 30 nM. The biotin-conjugated RNA complex was pulled down by incubating the cell lysates with streptavidin-coated magnetic beads (Life Technologies). The amount of cZBTB44 in … danfoss canking downloadWebMay 31, 2024 · The biotin-coupled miRNA capture assay was performed as previously described. 17 In brief, miR-206 mimics and miRNA negative control were labeled with biotin at the 3′ end and transiently transfected into LoVo and HCT116 cells. After 24 hrs of culture, the cells were harvested and lysed in a lysis buffer, followed by incubation with ... danfoss cf-rcWebDec 20, 2024 · Biotin-coupled miRNA capture. For biotin-coupled capture, approximately 5 × 10 6 BCa cells (T24 and 253 J-BV) were transfected with biotin-miR135a-5p or negative control (NC) by … danfoss central heating timersWebJun 21, 2024 · In addition, luciferase reporter, biotin‑coupled miRNA capture and RNA immunoprecipitation assays revealed that circZYG11B could regulate RGC function through circZYG11B/microRNA‑620/PTEN signaling. Clinically, RT‑qPCR assays demonstrated that circZYG11B expression was markedly increased in the aqueous humor of patients with … birmingham housing authority mccoy buildingWebJan 1, 2016 · Therefore, to identify all miR-96 targets (the miR-96 targetome) in an unbiased manner, we undertook a biotin-miRNA (bi-miR) capture and streptavidin pulldown … birmingham housing authority phoneWebNov 23, 2024 · Biotin-coupled miRNA capture, fluorescence in situ hybridization and luciferase reporter assays were conducted to confirm the relationship between circMMP9 and miR-124. In this study, we screened differentially expressed circRNAs and identified circMMP9 in GBM. We found that circMMP9 acted as an oncogene, was upregulated in … birmingham housing authority propertiesWebIn the biotin-coupled miRNA capture assay, the direct interaction between HIPK3 and miR-194 was verified. While upregulating HIPK3 promoted NSCLC cell proliferation, co-transfection with both HIPK3 and miR-194 abrogated the tumor-promoting effects of HIPK3. Altogether, these data corroborated that HIPK3 and miR-149 exert opposing roles in … danfoss controller software